HomeFAQsWhat is the maximum binding capacity of the of the columns in the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
FAQ: What is the maximum binding capacity of the of the columns in the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110)?
The matrix supplied in each column can bind up to 20 µg of purified DNA. However, it is important to note that performing a miniprep introduces cellular components such as carbohydrates, proteins, and residual nucleic acid from the host, all of which can affect the binding efficiency of the plasmid DNA to the matrix.
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