Appendix A for use with NEBNext Ultra II RNA First Strand Synthesis Module (E7771)
Note: These recommendations have been optimized using Universal Human Reference Total RNA. Other types of RNA may require different fragmentation times.
![](/~/media/Catalog/All-Protocols/D89874669A5349F0BF0D9CD499FD8B01/Content/Figure_6_1a.jpg)
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.