Protocol for RNA (E7445)
![](/~/media/Catalog/All-Protocols/53F8EEE3C51D46A78D1C199F935EF9FA/Content/caution.jpg)
Protocol
Perform Adaptor Ligation- Add the following components directly to the End Prep reaction mixture
(65 μl) and mix well:
Blunt/TA Ligase Master Mix 15 μl
NEBNext Adaptor (15 μM)* 1 μl
Nuclease-free Water 2.5 μl
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Total volume 83.5 μl
*The adaptor is provided in NEBNext Singleplex (NEB #E7350) or NEBNext Multiplex (NEB #E7335, #E7500) Oligos for Illumina. - Mix by pipetting, followed by a quick spin to collect all liquid from the sides of the tube.
- Incubate 15 minutes at 20°C.
Note: USER step is performed during the PCR reaction. - Proceed to desired size selection method.