FAQ: Are NEB's endoglycosidases compatible with protease inhibitor cocktails?
Aprotinin (10 µg/ml final concentration), Benzamidine (1 mM final concentration), Pepstatin (10 µg/ml final concentration), Leupeptin (1 µM final concentration), EGTA (1 mM final concentration), EDTA (1 mM final concentration), PMSF (1 mM final concentration). Note: PMSF is not highly recommended as it has the ability to modify basic residues on glycoprotein substrates.