HomeFAQsI observed high backgrounds with empty vector when using NEBridge Ligase Master Mix. What should I do?
FAQ: I observed high backgrounds with empty vector when using NEBridge Ligase Master Mix. What should I do?
We suggest performing the transformation immediately after the reaction is done. If you store the reactions at -20°C overnight or for an extensive period, you will need to do another 60°C incubation for 5 minutes right before the transformation. 60°C favors the restriction enzyme activity over T4 DNA Ligase activity and can reduce background by digesting any “re-ligated” vector that still carries the restriction enzyme site.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.