Protocol for use with TelN Protelomerase (M0651)
- Set up the following reaction in a microcentrifuge tube on ice.
COMPONENTS 20 μl of REACTION dsDNA (< 300 fmol of TelN sites) X μl ThermoPol Reaction Buffer (10X) 2 μl TelN Protelomerase 1 μl Nuclease-free water to 20 μl
- Gently mix the reaction by pipetting up and down and microcentrifuge briefly.
- Incubate at 30°C for 30 minutes followed by 75°C for 5 minutes.