Enzyme | Sequence | Recombinant | Time-Saver Qualified | Unit Assay Substrate | Plasmid Substrate | PCR Substrate |
---|---|---|---|---|---|---|
AatII | GACGT/C | ![]() |
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AccI | GT/MKAC | ![]() |
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Acc65I | G/GTACC | ![]() |
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AciI | CCGC(-3/-1) | ![]() |
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AclI | AA/CGTT | ![]() |
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AcuI | CTGAAG(16/14) | ![]() |
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AflII | C/TTAAG | ![]() |
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AgeI-HF® | A/CCGGT | ![]() |
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AhdI | GACNNN/NNGTC | ![]() |
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AluI | AG/CT | ![]() |
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AlwNI | CAGNNN/CTG | ![]() |
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ApaI | GGGCC/C | ![]() |
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ApaLI | G/TGCAC | ![]() |
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ApeKI | G/CWGC | ![]() |
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ApoI-HF | R/AATTY | ![]() |
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AscI | GG/CGCGCC | ![]() |
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NT | ||
AseI | AT/TAAT | ![]() |
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NT | ||
AvaI | C/YCGRG | ![]() |
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AvaII | G/GWCC | ![]() |
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AvrII | C/CTAGG | ![]() |
NT | NT | ||
BaeGI | GKGCM/C | ![]() |
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BaeI | (10/15)ACNNNNGTAYC(12/7) | ![]() |
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BamHI § | G/GATCC | ![]() |
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BamHI-HF® | G/GATCC | ![]() |
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BbsI § | GAAGAC(2/6) | ![]() |
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BbsI-HF® | GAAGAC(2/6) | ![]() |
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BbvI | GCAGC(8/12) | ![]() |
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BciVI | GTATCC(6/5) | ![]() |
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BclI § | T/GATCA | ![]() |
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BclI-HF | T/GATCA | ![]() |
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BcoDI | GTCTC(1/5) | ![]() |
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BfuAI | ACCTGC(4/8) | ![]() |
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BglI | GCCNNNN/NGGC | ![]() |
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BglII | A/GATCT | ![]() |
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BlpI | GC/TNAGC | ![]() |
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BmgBI | CACGTC(-3/-3) | ![]() |
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BmtI-HF® | GCTAG/C | ![]() |
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BpuEI | CTTGAG(16/14) | ![]() |
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BsaAI | YAC/GTR | ![]() |
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BsaHI | GR/CGYC | ![]() |
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BsaI-HF®v2 | GGTCTC(1/5) | ![]() |
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BsaWI | W/CCGGW | ![]() |
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BsaXI | (9/12)ACNNNNNCTCC(10/7) | ![]() |
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BseRI | GAGGAG(10/8) | ![]() |
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BsgI | GTGCAG(16/14) | ![]() |
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BsiEI | CGRY/CG | ![]() |
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BsiHKAI | GWGCW/C | ![]() |
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BsiWI § | C/GTACG | ![]() |
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BsiWI-HF® | C/GTACG | ![]() |
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BslI | CCNNNNN/NNGG | ![]() |
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BsmAI | GTCTC(1/5) | ![]() |
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BsmBI-v2 | CGTCTC | ![]() |
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BsmI | GAATGC(1/-1) | ![]() |
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BsoBI | C/YCGRG | ![]() |
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BspCNI | CTCAG(9/7) | ![]() |
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BspEI | T/CCGGA | ![]() |
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BspHI | T/CATGA | ![]() |
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Bsp1286I | GDGCH/C | ![]() |
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BspQI | GCTCTTC(1/4) | ![]() |
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BsrBI | CCGCTC(-3/-3) | ![]() |
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BsrDI | GCAATG(2/0) | ![]() |
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BsrFI-v2 | R/CCGGY | ![]() |
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BsrGI-HF® | T/GTACA | ![]() |
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BsrI | ACTGG(1/-1) | ![]() |
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BssHII | G/CGCGC | ![]() |
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BssSI-v2 | CACGAG(-5/-1) | ![]() |
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BstBI | TT/CGAA | ![]() |
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BstEII-HF® | G/GTNACC | ![]() |
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BstNI | CC/WGG | ![]() |
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BstUI | CG/CG | ![]() |
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BstXI | CCANNNNN/NTGG | ![]() |
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BstYI | R/GATCY | ![]() |
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BstZ17I-HF® | GTATAC | ![]() |
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Bsu36I | CC/TNAGG | ![]() |
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BtgI | C/CRYGG | ![]() |
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BtsCI | GGATG(2/0) | ![]() |
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BtsI-v2 | GCAGTG(2/0) | ![]() |
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Cac8I | GCN/NGC | ![]() |
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ClaI | AT/CGAT | ![]() |
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CspCI | (11/13)CAANNNNNGTGG(12/10) | ![]() |
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CviQI | G/TAC | ![]() |
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DdeI | C/TNAG | ![]() |
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DpnI | GA/TC | ![]() |
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DpnII | /GATC | ![]() |
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DraI | TTT/AAA | ![]() |
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DraIII-HF® | CACNNN/GTG | ![]() |
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DrdI | GACNNNN/NNGTC | ![]() |
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EagI-HF® | C/GGCCG | ![]() |
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EarI | CTCTTC(1/4) | ![]() |
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Eco53kI | GAG/CTC | ![]() |
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EcoNI | CCTNN/NNNAGG | ![]() |
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EcoO109I | RG/GNCCY | ![]() |
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EcoP15I | CAGCAG(25/27) | ![]() |
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EcoRI § | G/AATTC | ![]() |
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EcoRI-HF® | G/AATTC | ![]() |
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EcoRV § | GAT/ATC | ![]() |
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EcoRV-HF® | GAT/ATC | ![]() |
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Esp3I | CGTCTC(1/5) | ![]() |
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Fnu4HI | GC/NGC | ![]() |
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FseI | GGCCGG/CC | ![]() |
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FspI | TGC/GCA | ![]() |
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HaeII | RGCGC/Y | ![]() |
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HaeIII | GG/CC | ![]() |
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HhaI | GCG/C | ![]() |
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HincII | GTY/RAC | ![]() |
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HindIII-HF® | A/AGCTT | ![]() |
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HinfI | G/ANTC | ![]() |
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HinP1I | G/CGC | ![]() |
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HpaII | C/CGG | ![]() |
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HphI | GGTGA(8/7) | ![]() |
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HpyAV | CCTTC(6/5) | ![]() |
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NT | ||
HpyCH4IV | A/CGT | ![]() |
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HpyCH4V | TG/CA | ![]() |
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Hpy166II | GTN/NAC | ![]() |
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KpnI-HF® | GGTAC/C | ![]() |
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MboI | /GATC | ![]() |
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MboII | GAAGA(8/7) | ![]() |
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MfeI-HF® | C/AATTG | ![]() |
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MluCI | /AATT | ![]() |
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MluI-HF® | A/CGCGT | ![]() |
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MlyI | GAGTC(5/5) | ![]() |
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MmeI | TCCRAC(20/18) | ![]() |
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MnlI | CCTC(7/6) | ![]() |
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MseI | T/TAA | ![]() |
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MslI | CAYNN/NNRTG | ![]() |
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MspA1I | CMG/CKG | ![]() |
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MspI | C/CGG | ![]() |
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MwoI | GCNNNNN/NNGC | ![]() |
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NciI | CC/SGG | ![]() |
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NcoI § | C/CATGG | ![]() |
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NcoI-HF® | C/CATGG | ![]() |
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NdeI | CA/TATG | ![]() |
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NgoMIV | G/CCGGC | ![]() |
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NheI-HF® | G/CTAGC | ![]() |
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NlaIII | CATG/ | ![]() |
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NotI § | GC/GGCCGC | ![]() |
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NotI-HF® | GC/GGCCGC | ![]() |
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NruI-HF® | TCG/CGA | ![]() |
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NsiI § | ATGCA/T | ![]() |
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NsiI-HF® | ATGCA/T | ![]() |
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NspI | RCATG/Y | ![]() |
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PacI | TTAAT/TAA | ![]() |
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PaeR7I | C/TCGAG | ![]() |
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PflFI | GACN/NNGTC | ![]() |
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PflMI | CCANNNN/NTGG | ![]() |
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PmeI | GTTT/AAAC | ![]() |
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NT | ||
PmlI | CAC/GTG | ![]() |
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PpuMI | RG/GWCCY | ![]() |
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PshAI | GACNN/NNGTC | ![]() |
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PsiI-v2 | TTA/TAA | ![]() |
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PstI § | CTGCA/G | ![]() |
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PstI-HF® | CTGCA/G | ![]() |
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PvuI-HF® | CGAT/CG | ![]() |
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PvuII § | CAG/CTG | ![]() |
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PvuII-HF® | CAG/CTG | ![]() |
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RsaI | GT/AC | ![]() |
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SacI-HF® | GAGCT/C | ![]() |
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SacII | CCGC/GG | ![]() |
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SalI § | G/TCGAC | ![]() |
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SalI-HF® | G/TCGAC | ![]() |
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SapI | GCTCTTC(1/4) | ![]() |
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SbfI-HF® | CCTGCA/GG | ![]() |
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ScaI-HF® | AGT/ACT | ![]() |
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SfiI | GGCCNNNN/NGGCC | ![]() |
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SfoI | GGC/GCC | ![]() |
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SmaI | CCC/GGG | ![]() |
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SpeI-HF® | A/CTAGT | ![]() |
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SphI-HF® | GCATG/C | ![]() |
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SrfI | GCCC/GGGC | ![]() |
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SspI-HF® | AAT/ATT | ![]() |
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StuI | AGG/CCT | ![]() |
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StyD4I | /CCNGG | ![]() |
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StyI-HF® | C/CWWGG | ![]() |
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SwaI | ATTT/AAAT | ![]() |
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TaqI-v2 | T/CGA | ![]() |
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TfiI | G/AWTC | ![]() |
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TseI | G/CWGC | ![]() |
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TspMI | C/CCGGG | ![]() |
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TspRI | NNCASTGNN/ | ![]() |
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Tth111I | GACN/NNGTC | ![]() |
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XbaI | T/CTAGA | ![]() |
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XhoI | C/TCGAG | ![]() |
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XmaI | C/CCGGG | ![]() |
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XmnI | GAANN/NNTTC | ![]() |
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§ An HF version of this enzyme is available.
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