Protocol for XRN-1 NEB M0338

1. Set-up the reaction as follows:



COMPONENTS	20 μl REACTION
Single stranded 5’phos-RNA	1μg
NEBuffer 3 (10X)	2 μl (1X)
Nuclease-free H2O (NEB #B1500)	up to 20 μl
XRN-1 (1,000 U/ml)	2 μl

 
2. Mix thoroughly.
3. Incubate at 37°C for 60 minutes.