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Schildkraut Lab
Schildkraut Lab
Bioluminescence and coelenterazine sulfotransferase
Bioluminescence and coelenterazine sulfotransferase
We have cloned and expressed two full-length cDNAs that encode two distinct Renilla Coel-STs by combining de novo peptide sequencing with transcriptome analysis. These were both shown to convert coelenterazine sulfate to coelenterazine. The sulfotransferase is a member of the Pfam, PF00685. The recombinant form of Coel-ST now makes practicable a very sensitive PAP assay described by Stanley in 1975 and refined further by Anderson in 1978.
Detection of Gaussia luciferase expressed in E. Coli
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Pathway for conversion of coelenterazine sulfate to green light via Bioluminescense Resonance Energy Transfer in the soft coral Renilla