Perform End Repair/dA-tail of cDNA Library (E7420)
Protocol
- To the purified double-stranded cDNA (55.5 μl), add the following components:
NEBNext End Repair Reaction Buffer (10X) 6.5 μl
NEBNext End Prep Enzyme Mix 3 μl
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Total volume 65 μl
- Incubate the sample in a thermal cycler as follows:
30 minutes at 20°C
30 minutes at 65°C
Hold at 4°C
- Proceed immediately to Adaptor Ligation.