Buffer Preparation Guidance for Monarch^® Spin PCR & DNA Cleanup Kit (5 µl) (NEB #T1130)

Add isopropanol to the Monarch Buffer BZ prior to use (0.43 volume of isopropanol per volume of Buffer BZ).

• For T1130S (50-prep) kit, add 18 ml of isopropanol to Monarch Buffer BZ.
• For T1130L (250-prep) kit, add 72 ml of isopropanol to Monarch Buffer BZ.

Add ethanol to the Monarch Buffer WZ prior to use (4 volumes of ≥ 95% ethanol per volume of Buffer WZ).

• For T1130S (50-prep) kit, add 20 ml of ethanol to Buffer WZ.
• For T1130L (250-prep) kit, add 104 ml of ethanol to Buffer WZ.

Always keep all buffer bottles tightly closed when not actively in use.