FAQ: What are the main causes of blunting reaction failure?

The following conditions can cause exonuclease activity to overwhelm the polymerase activity, causing recessed instead of blunt ends:

• Failure to add nucleotides, or the nucleotide concentration is too low
• Adding too much enzyme
• Incubating for too long
• Incubating at temperatures greater than the recommended temperature
• Heat inactivating without EDTA
  • FAQ: Can this polymerase be heat inactivated?

Please see the recommended blunting protocol for 

• DNA Polymerase I, Large (Klenow) Fragment by clicking here.
• T4 DNA Polymerase by clicking here.