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Protocol Search

Search from over 1,000 NEB protocols within our database.

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The message is spreading.
NEB has everything you need for your RNA-related workflows.
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NEBNext UltraShear® Long Read
Fast enzymatic gDNA fragmentation for long-read sequencing, tunable from 2 - 30 kb, while preserving methylation markers. Automation-friendly, broad inputs, boosts yield for ONT/PacBio, reliable, cost-effective.
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Q5®-XT Hot Start High-Fidelity 2X Master Mix
High‑fidelity (~280× Taq) polymerase enables fast amplification of diverse targets up to 20 kb, with a hot-start setup, short extension times, and a 62°C universal annealing protocol.
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EnClose™ Cell-free dbDNA™ Synthesis Kit
Fast, fermentation‑free enzymatic DNA manufacturing delivers high‑purity, backbone‑free dbDNA lacking bacterial sequences, simplifying regulation, improving safety, and supporting sensitive mRNA workflows.
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Review NEB’s Latest Research

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Feature Article

This article traces molecular cloning’s history and progress, highlighting how improved enzymes, assembly methods, and DNA design tools have advanced cloning efficiency, precision and applications.

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Featured Video

The NEBaseChanger® Primer Design Tool Tutorial video will explain how to design primers for site-directed and multi-site mutagenesis using NEBaseChanger. This tool is ideal for NEB Q5® and NEBuilder® HiFi DNA Assembly applications.

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Learn about our one-day in vitro workflow of Golden Gate Assembly, RCA, and cell-free protein expression for high-throughput screening, generating custom protein variants.

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Application Note

Read about the benefits and considerations of using a single, universal annealing temperature in PCR with various high-fidelity products.

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Web Tool

The latest version of NEBaseChanger® is available to help design site-directed mutagenesis primers. Compatible with ambiguous bases, substitute based on amino acid position, batch upload for multiple primer sets and add mutations to primer tails.

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New Publication

Explore NEB Expressions, featuring a precision medicine case study from discovery to targeted therapy, cell-free dbDNA synthesis, enzymatic solutions for circular RNA enrichment, conservation efforts in Costa Rica, RNA workflow products and the 2026 Passion in Science Awards.

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Featured Podcast

Interview with John Burns: Unravelling Marine Mysteries

Featured Podcast

Interview with John Burns: Unravelling Marine Mysteries

John Burns of Bigelow Laboratory for Ocean Sciences shares how he thinks about the information that drives biology.

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Featured Video

Generating dbDNA™ using the EnClose™ Cell-free dbDNA Synthesis Kit

Featured Video

Generating dbDNA™ using the EnClose™ Cell-free dbDNA Synthesis Kit

This animation demonstrates a one-day workflow for synthesizing dbDNA (doggybone™ DNA) using the EnClose™ Cell-free dbDNA Synthesis Kit.

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Featured Webinar

Accelerating RNA & DNA Therapeutics with Cell-free DNA Synthesis

Featured Webinar

Accelerating RNA & DNA Therapeutics with Cell-free DNA Synthesis

Learn about cell-free DNA synthesis for RNA and DNA therapeutics with a focus on closed-ended DNA inputs and an overview of the recent launch of our new EnClose™ Cell-free dbDNA Synthesis Kit.

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